Human Anti-SIRPA Recombinant Antibody (TAB-453CT)
CAT#: TAB-453CT
This product is a recombinant human antibody that recognizes SIRPA. The antibody was purified by affinity chromatography.
Tested Data
Gene Expression
WB
Figure 1 Anti-Human SIRPA Antibody (TAB-453CT) in WB
Western blot analysis of TAB-453CT was performed by loading Human SIRPA Protein (His Tag) in reduced condition Lane 1, Lane 2, Lane 3 (0.1 μg 0.3 μg 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-453CT (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.
DB
Figure 2 Anti-Human SIRPA Antibody (TAB-453CT) in DB
Dot Blot analysis of TAB-453CT was performed by coating with Human SIRPA Protein (His Tag).
TAB-453CT incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000)
ELISA
Figure 3 Anti-Human SIRPA Antibody (TAB-453CT) in ELISA
ELISA analysis of TAB-453CT was performed by coating with Human SIRPA Protein (His Tag). Then blocked with BSA and incubated with TAB-453CT. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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Normal Tissue
Figure 1 Cerebral cortex
Neuropil
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2776/6761_B_8_5.jpg
Normal Tissue
Figure 2 Colon
Endocrine cells
Staining:Medium
Intensity: Moderate
Enterocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2776/6761_A_7_3.jpg
Normal Tissue
Figure 3 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2776/6761_A_8_8.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (fHDF/TERT166, RPTEC TERT1, SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198053-SIRPA
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Specifications
- Host Species
- Human
- Type
- Chimeric (mouse/human) IgG4
- Specificity
- Human
- Species Reactivity
- Human
- Applications
- Cyt, IF, FC
- Related Disease
- A wide range of human cancers
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- SIRPA; signal-regulatory protein alpha; BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1; tyrosine-protein phosphatase non-receptor type substrate 1; myd-1 antigen; inhibitory receptor SHPS-1; macrophage fusion receptor; CD172 antigen-like family member A; tyrosine phosphatase SHP substrate 1; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; anti-SIRPA
- Gene ID
- 140885
- UniProt ID
- P78324
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Enhances Immunotherapy Exploration
Our lab has seen increased clarity in studying immune checkpoint pathways using this antibody. It provides crucial insights into SIRPA interactions, opening pathways for potential cancer immunotherapies that are now being investigated further.
Robust and Versatile
We've applied this antibody across various assay platforms with robust performance. Its versatility in applications from immunofluorescence to flow cytometry supports a broad range of experimental needs in our lab.
Ideal for Cancer Research
This antibody has been ideal for exploring SIRPA's role in cancer. It provides clarity in studying immune evasion strategies by tumors, crucial for designing immune-based interventions with therapeutic potential.
Q&As
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What specific targets does this antibody bind to?
A: The antibody specifically binds to the SIRPα protein on myeloid cells. This specificity aids researchers in exploring the immune checkpoint pathways, as SIRPα is pivotal in the "don't-eat-me" signal mechanism exploited by tumors.
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What makes the sensitivity and specificity of this antibody significant?
A: The recombinantly engineered antibody ensures high sensitivity and specificity, crucial for detecting and quantifying SIRPα without cross-reactivity, thus enhancing experimental accuracy in complex biological matrices.
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How stable is this antibody, and what are the storage recommendations?
A: The antibody is quite stable, with recommended storage at 4°C for short-term and -20°C for long-term. Care should be taken to avoid multiple freeze-thaw cycles to maintain its structural integrity and functionality.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-453CT, RRID: AB_3111953)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3950z | Mouse Anti-SIRPA Recombinant Antibody (clone 43H3) | WB, FC, ICC | Mouse IgG2b, κ |
| MOB-1381CT | Recombinant Mouse anti-Rat SIRPA Monoclonal antibody (FE0) | FC, IHC-Fr, IP, WB | |
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| VS3-FY1336 | Recombinant Rabbit Anti-SIRPA Antibody (clone R04-7G6) | WB | Rabbit IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-454CT | Anti-Human SIRPA Recombinant Antibody (AM4-1) | ELISA | Human antibody |
| TAB-454CT-S(P) | Anti-Human SIRPA Recombinant Antibody scFv Fragment (AM4-1) | ELISA | Human antibody |
| TAB-454CT-F(E) | Anti-Human SIRPA Recombinant Antibody Fab Fragment (AM4-1) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-453CT-S(P) | Human Anti-SIRPA Recombinant Antibody; scFv Fragment (TAB-453CT-S(P)) | WB, ELISA | Human scFv |
| TAB-453CT-F(E) | Human Anti-SIRPA Recombinant Antibody; Fab Fragment (TAB-453CT-F(E)) | WB, ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0465MZ | Chicken Anti-PTPNS1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1970CQ | Recombinant Rat Anti-Sirpa Antibody (P84) | FC, BL, IF, IHC, IP | IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1971CQ | Recombinant Mouse Anti-Sirpa Antibody (CBL650) | WB, FC, CyTOF, Neut | IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4429 | Hi-Affi™ Recombinant Rabbit Anti-SIRPA Monoclonal Antibody (SI522DS) | ELISA | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0023-LSX-S(P) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM4-1); scFv Fragment | ELISA | Humanized scFv |
| HPAB-N0386-YC-S(P) | Human Anti-SIRPA Recombinant Antibody; scFv Fragment (HPAB-N0386-YC-S(P)) | ELISA, FC | Human scFv |
| HPAB-N0387-YC-S(P) | Mouse Anti-SIRPA Recombinant Antibody; scFv Fragment (HPAB-N0387-YC-S(P)) | ELISA, FC | Mouse scFv |
| HPAB-N0388-YC-S(P) | Mouse Anti-SIRPA Recombinant Antibody; scFv Fragment (HPAB-N0388-YC-S(P)) | ELISA, FC | Mouse scFv |
| HPAB-2559LY-S(P) | Human Anti-SIRPA Recombinant Antibody; scFv Fragment (HPAB-2559LY-S(P)) | Inhib | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0023-LSX-F(E) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM4-1); Fab Fragment | ELISA | Humanized Fab |
| HPAB-0024-LSX-F(E) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM3-4); Fab Fragment | ELISA | Humanized Fab |
| HPAB-0025-LSX-F(E) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM4-5); Fab Fragment | ELISA | Humanized Fab |
| PABJ-0084-F(E) | Human Anti-SIRPA Recombinant Antibody Fab Fragment (PABJ-0084-F(E)) | ELISA, Block | Human Fab |
| PABJ-0085-F(E) | Human Anti-SIRPA Recombinant Antibody Fab Fragment (PABJ-0085-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-080CN | Human Anti-SIRPA Recombinant Antibody (clone 29-AM3-35) | ELISA, Inhib, FuncS | Human IgG |
| NS-080CN-F(E) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM3-35); Fab Fragment | ELISA | Human Fab |
| NS-080CN-S(P) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM3-35); scFv Fragment | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1462 | AbPlus™ Anti-SIRPA Magnetic Beads (VS-0724-YC1462) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY86 | Human Anti-SIRPA (clone 29-AM3-35) scFv-Fc Chimera | ELISA, Inhib | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC143 | Recombinant Anti-SIRPA Vesicular Antibody, EV Displayed (VS-0425-YC143) | Cyt, ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6598 | Anti-SIRPA Immunohistochemistry Kit | IHC | |
| VS-0525-XY6599 | Anti-Mouse SIRPA Immunohistochemistry Kit | IHC | |
| VS-0525-XY6600 | Anti-Rat SIRPA Immunohistochemistry Kit | IHC |
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